A72 MECHANISMS DRIVING FIBROSIS: Lactate Dehydrogenase Expression And Activity Is Altered In Alveolar Type Ii Cells From Patients With Idiopathic Pulmonary Fibrosis

Rationale: Idiopathic pulmonary fibrosis (IPF) is a devastating, chronic and progressive disease characterized by extensive lung scarring and eventual loss of pulmonary function. Methods: Human and murine primary ATII cells from normal or fibrotic lungs (IPF patients or bleomycin-treated mice) were purified and analyzed for lactate dehydrogenase (LDH) expression (mRNA by qPCR; protein by non-denaturing gel electrophoresis) and activity (extracellular flux and biochemical assays).