Polymorphism of gene apolipoprotein B in patients with chronic pancreatitis, combined with obesity and diabetes mellitus type 2

Polymorphism of gene apolipoprotein B in patients with chronic pancreatitis, combined with obesity and diabetes mellitus type 2

The purpose of research: To analyze the Ins / Del - polymorphism of Apo -B (rs17240441) in the structure of patients with chronic pancreatitis (CP), with comorbidity with type 2 diabetes mellitus and obesity. Materials and methods: The study of 90 participants who were divided into 2 groups. The fir...

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Veröffentlicht in:The pharma innovation 2016-08, Vol.5 (8, Part A), p.1
Hauptverfasser: Ferfetska, K V, OI, Fediv, Sydorchuk, L P
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Deoxyribonucleic acid
Diabetes
DNA
Obesity
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description The purpose of research: To analyze the Ins / Del - polymorphism of Apo -B (rs17240441) in the structure of patients with chronic pancreatitis (CP), with comorbidity with type 2 diabetes mellitus and obesity. Materials and methods: The study of 90 participants who were divided into 2 groups. The first group consisted of 49 patients with CP in combination with obesity and type 2 diabetes, the second - almost 41 healthy individuals (control group). Genomic DNA was isolated from peripheral blood using the test - systems "innu PREP Blood DNA Mini Kit (Germany). To determine the polymorphic variants of the gene ApoB ins / del using the PCR method. The distribution of genotypes and allele frequency was assessed χ2 test, according to the law of distribution of genotypes Hardy - Weinberg. Results and discussion: Analysis of the multiplicative model of inheritance revealed that I- allele is more common than "minor" D- allele at 40, 82% (χ2 = 32, 65, p
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Materials and methods: The study of 90 participants who were divided into 2 groups. The first group consisted of 49 patients with CP in combination with obesity and type 2 diabetes, the second - almost 41 healthy individuals (control group). Genomic DNA was isolated from peripheral blood using the test - systems "innu PREP Blood DNA Mini Kit (Germany). To determine the polymorphic variants of the gene ApoB ins / del using the PCR method. The distribution of genotypes and allele frequency was assessed χ2 test, according to the law of distribution of genotypes Hardy - Weinberg. Results and discussion: Analysis of the multiplicative model of inheritance revealed that I- allele is more common than "minor" D- allele at 40, 82% (χ2 = 32, 65, p&lt;0,001) in the experimental group and 21, 96% (χ2 = 7, 90, p = 0,005) in the control. In total, the surveyed population of 180 selected alleles dominated "wild" I - allele of the mutant to 32, 22% (p&lt;0,001). The distribution of genotypes in the cohort studied law consistent with Hardy - Weinberg (χ2 = 0, 25, p = 0, 62). Analysis of the overall co-dominant inheritance pattern, showed a significant advantage relative frequency InsIns- and InsDel-genotypes of the gene Apo-B over DelDel-genotype as that of persons investigated and the control groups. Conclusions: 1. Thus, the deletion of the functional portion of the DNA of the gene Apo-B (id:. Rs17240441) in the homozygous state in the second pair 2r23-24 chromosomes found in the studied population of North Bukovina in 13, 33% of cases: among patients with CP, type 2 diabetes and obesity - to 10,20%, among control group - in 17,07% of cases (p&gt;0,05). 2. InsIns-gene genotype Apo-B is found in every second patient HP, type 2 diabetes with obesity (51, 02%), which is 12, 0% more than in the control group (χ2 = 3, 95, p = 0, 047).</description><identifier>EISSN: 2277-7695</identifier><language>eng</language><publisher>New Delhi: The Pharma Innovation</publisher><subject>Deoxyribonucleic acid ; Diabetes ; DNA ; Obesity</subject><ispartof>The pharma innovation, 2016-08, Vol.5 (8, Part A), p.1</ispartof><rights>Copyright The Pharma Innovation Aug 2016</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Ferfetska, K V</creatorcontrib><creatorcontrib>OI, Fediv</creatorcontrib><creatorcontrib>Sydorchuk, L P</creatorcontrib><title>Polymorphism of gene apolipoprotein B in patients with chronic pancreatitis, combined with obesity and diabetes mellitus type 2</title><title>The pharma innovation</title><description>The purpose of research: To analyze the Ins / Del - polymorphism of Apo -B (rs17240441) in the structure of patients with chronic pancreatitis (CP), with comorbidity with type 2 diabetes mellitus and obesity. Materials and methods: The study of 90 participants who were divided into 2 groups. The first group consisted of 49 patients with CP in combination with obesity and type 2 diabetes, the second - almost 41 healthy individuals (control group). Genomic DNA was isolated from peripheral blood using the test - systems "innu PREP Blood DNA Mini Kit (Germany). To determine the polymorphic variants of the gene ApoB ins / del using the PCR method. The distribution of genotypes and allele frequency was assessed χ2 test, according to the law of distribution of genotypes Hardy - Weinberg. Results and discussion: Analysis of the multiplicative model of inheritance revealed that I- allele is more common than "minor" D- allele at 40, 82% (χ2 = 32, 65, p&lt;0,001) in the experimental group and 21, 96% (χ2 = 7, 90, p = 0,005) in the control. In total, the surveyed population of 180 selected alleles dominated "wild" I - allele of the mutant to 32, 22% (p&lt;0,001). The distribution of genotypes in the cohort studied law consistent with Hardy - Weinberg (χ2 = 0, 25, p = 0, 62). Analysis of the overall co-dominant inheritance pattern, showed a significant advantage relative frequency InsIns- and InsDel-genotypes of the gene Apo-B over DelDel-genotype as that of persons investigated and the control groups. Conclusions: 1. Thus, the deletion of the functional portion of the DNA of the gene Apo-B (id:. Rs17240441) in the homozygous state in the second pair 2r23-24 chromosomes found in the studied population of North Bukovina in 13, 33% of cases: among patients with CP, type 2 diabetes and obesity - to 10,20%, among control group - in 17,07% of cases (p&gt;0,05). 2. InsIns-gene genotype Apo-B is found in every second patient HP, type 2 diabetes with obesity (51, 02%), which is 12, 0% more than in the control group (χ2 = 3, 95, p = 0, 047).</description><subject>Deoxyribonucleic acid</subject><subject>Diabetes</subject><subject>DNA</subject><subject>Obesity</subject><issn>2277-7695</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNjcFqwzAQREWh0NDmHxZybSCVnRpdW1J6zKH3INubeIOsVbRrgk_99QrSD8gcZmDewDyYhbVNs27e3fbJLEXOmyLnqsq6hfndc5hHzmkgGYGPcMKI4BMHSpwyK1KEDyiWvBJGFbiSDtANmSN1pY1dxoKU5BU6HluK2N823KKQzuBjDz35FhUFRgyBdBLQOSHYF_N49EFw-Z_PZvW1-_n8Xpfvy4SihzNPORZ0eHO2rreuqavqvtUfdpBRQg</recordid><startdate>20160801</startdate><enddate>20160801</enddate><creator>Ferfetska, K V</creator><creator>OI, Fediv</creator><creator>Sydorchuk, L P</creator><general>The Pharma Innovation</general><scope>04Q</scope><scope>04S</scope><scope>04W</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20160801</creationdate><title>Polymorphism of gene apolipoprotein B in patients with chronic pancreatitis, combined with obesity and diabetes mellitus type 2</title><author>Ferfetska, K V ; OI, Fediv ; Sydorchuk, L P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_19244597433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Deoxyribonucleic acid</topic><topic>Diabetes</topic><topic>DNA</topic><topic>Obesity</topic><toplevel>online_resources</toplevel><creatorcontrib>Ferfetska, K V</creatorcontrib><creatorcontrib>OI, Fediv</creatorcontrib><creatorcontrib>Sydorchuk, L P</creatorcontrib><collection>India Database</collection><collection>India Database: Business</collection><collection>India Database: Science &amp; Technology</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>The pharma innovation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferfetska, K V</au><au>OI, Fediv</au><au>Sydorchuk, L P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymorphism of gene apolipoprotein B in patients with chronic pancreatitis, combined with obesity and diabetes mellitus type 2</atitle><jtitle>The pharma innovation</jtitle><date>2016-08-01</date><risdate>2016</risdate><volume>5</volume><issue>8, Part A</issue><spage>1</spage><pages>1-</pages><eissn>2277-7695</eissn><abstract>The purpose of research: To analyze the Ins / Del - polymorphism of Apo -B (rs17240441) in the structure of patients with chronic pancreatitis (CP), with comorbidity with type 2 diabetes mellitus and obesity. Materials and methods: The study of 90 participants who were divided into 2 groups. The first group consisted of 49 patients with CP in combination with obesity and type 2 diabetes, the second - almost 41 healthy individuals (control group). Genomic DNA was isolated from peripheral blood using the test - systems "innu PREP Blood DNA Mini Kit (Germany). To determine the polymorphic variants of the gene ApoB ins / del using the PCR method. The distribution of genotypes and allele frequency was assessed χ2 test, according to the law of distribution of genotypes Hardy - Weinberg. Results and discussion: Analysis of the multiplicative model of inheritance revealed that I- allele is more common than "minor" D- allele at 40, 82% (χ2 = 32, 65, p&lt;0,001) in the experimental group and 21, 96% (χ2 = 7, 90, p = 0,005) in the control. In total, the surveyed population of 180 selected alleles dominated "wild" I - allele of the mutant to 32, 22% (p&lt;0,001). The distribution of genotypes in the cohort studied law consistent with Hardy - Weinberg (χ2 = 0, 25, p = 0, 62). Analysis of the overall co-dominant inheritance pattern, showed a significant advantage relative frequency InsIns- and InsDel-genotypes of the gene Apo-B over DelDel-genotype as that of persons investigated and the control groups. Conclusions: 1. Thus, the deletion of the functional portion of the DNA of the gene Apo-B (id:. Rs17240441) in the homozygous state in the second pair 2r23-24 chromosomes found in the studied population of North Bukovina in 13, 33% of cases: among patients with CP, type 2 diabetes and obesity - to 10,20%, among control group - in 17,07% of cases (p&gt;0,05). 2. InsIns-gene genotype Apo-B is found in every second patient HP, type 2 diabetes with obesity (51, 02%), which is 12, 0% more than in the control group (χ2 = 3, 95, p = 0, 047).</abstract><cop>New Delhi</cop><pub>The Pharma Innovation</pub></addata></record>
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subjects Deoxyribonucleic acid
Diabetes
DNA
Obesity
title Polymorphism of gene apolipoprotein B in patients with chronic pancreatitis, combined with obesity and diabetes mellitus type 2
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