Polymorphism of gene apolipoprotein B in patients with chronic pancreatitis, combined with obesity and diabetes mellitus type 2

The purpose of research: To analyze the Ins / Del - polymorphism of Apo -B (rs17240441) in the structure of patients with chronic pancreatitis (CP), with comorbidity with type 2 diabetes mellitus and obesity. Materials and methods: The study of 90 participants who were divided into 2 groups. The first group consisted of 49 patients with CP in combination with obesity and type 2 diabetes, the second - almost 41 healthy individuals (control group). Genomic DNA was isolated from peripheral blood using the test - systems "innu PREP Blood DNA Mini Kit (Germany). To determine the polymorphic variants of the gene ApoB ins / del using the PCR method. The distribution of genotypes and allele frequency was assessed χ2 test, according to the law of distribution of genotypes Hardy - Weinberg. Results and discussion: Analysis of the multiplicative model of inheritance revealed that I- allele is more common than "minor" D- allele at 40, 82% (χ2 = 32, 65, p