Investigation of Association Between Paraoxonase-1 L55M (RS854560) and Q192R (RS662) Polymorphisms and Potential Atherosclerotic Risk Factors in PCOS Patients

The aim of this study was to examine the frequency of potential atherosclerotic risk markers Q192R (rs662) and L55M (rs854560) in patients with PCOS and control group in Turkish women. Blood samples were collected from 151 patients with PCOS and 52 healthy women at Recep Tayyip Erdoğan University Hospital, Rize. All individuals underwent an evaluation of clinical examination and transabdominal ultrasound. Genomic DNA was extracted from the samples. RFLP method was performed following the amplifications of the target regions. The L55M and Q192R polymorphisms were detected by AlwI and NlaIII digestion, respectively. The genotyping results of 88 samples of this cohort were also confirmed by DNA sequencing. Patients with 192QR/192RR genotypes had a 2.5-time higher risk of representing PCOS compared to the individuals with 192QQ genotype. Differences in genotype and allele frequencies of PON1-55 were not found to be significant. Q192R (AUC: 0.613, p=0.017) and BMI (0.618, p=0.012) were established to be significant predictors of PCOS in a model including fasting glucose, insulin, HOMA-IR, total cholesterol, triglyceride, and LDL/HDL ratio as covariates (AUC: 0.655, p=0.001). Q192R was more strongly correlated with PCOS than previously suggested atherosclerotic risk factors, BMI, metabolic syndrome (MetS), and insulin resistance (IR). Q192R discriminates the patients with PCOS from controls significantly. Although further studies are needed, we suggest that individuals carrying an R allele are at a higher risk of developing PCOS in Turkish women. Q192R may be suggested to be a surrogate and robust predictive marker for the risk of developing PCOS and atherosclerosis in individuals.