TIMELESS Confers Cisplatin Resistance in Nasopharyngeal Carcinoma by Activating the Wnt/β-catenin Signaling Pathway and Promoting the Epithelial Mesenchymal Transition

Abstract This study investigated the expression, clinicopathological significance and mechanism of action of TIMELESS, a mammalian homolog of a Drosophila circadian rhythm gene, in nasopharyngeal carcinoma. Quantitative real-time PCR, western blotting and immunohistochemistry revealed TIMELESS was u...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Cancer letters 2017-08, Vol.402, p.117-130
Hauptverfasser:	Liu, Sai-Lan, Lin, Huan-Xin, Lin, Chu-Yong, Sun, Xiao-Qing, Ye, Li-Ping, Qiu, Fang, Wen, Wen, Hua, Xin, Wu, Xian-Qiu, Li, Jun, Song, Li-Bing, Guo, Ling
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Animals Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Biopsy Breast cancer Carcinoma - drug therapy Carcinoma - genetics Carcinoma - metabolism Carcinoma - pathology Cell cycle Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Cell division Cell Line, Tumor Chemotherapy Circadian rhythm Circadian rhythms Cisplatin Cisplatin - pharmacology Colorectal cancer Cytotoxicity Deoxyribonucleic acid Disease-Free Survival DNA Dose-Response Relationship, Drug Drug resistance Drug Resistance, Neoplasm Epithelial-Mesenchymal Transition - drug effects Epithelial–mesenchymal transition Female Fruit flies Gene expression Gene Expression Regulation, Neoplastic Hematology, Oncology and Palliative Medicine Homology Humans Immunohistochemistry Intracellular Signaling Peptides and Proteins - genetics Intracellular Signaling Peptides and Proteins - metabolism Kaplan-Meier Estimate Liver cancer Lung cancer Male Mesenchyme Metastases Metastasis Mice, Inbred BALB C Mice, Nude Middle Aged Multivariate analysis Nasopharyngeal Carcinoma Nasopharyngeal Neoplasms - drug therapy Nasopharyngeal Neoplasms - genetics Nasopharyngeal Neoplasms - metabolism Nasopharyngeal Neoplasms - pathology Neoplasm Grading Neoplasm Staging Ovarian cancer Paraffin Phenotype Polyclonal antibodies Polymerase chain reaction Proportional Hazards Models RNA Interference Signal transduction Survival Throat cancer Time Factors TIMELESS Timeless protein Transcription Transcription, Genetic Transfection Tumor cells Tumors Western blotting Wnt protein Wnt Signaling Pathway - drug effects Wnt/β-catenin pathway Xenograft Model Antitumor Assays β-Catenin
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Abstract This study investigated the expression, clinicopathological significance and mechanism of action of TIMELESS, a mammalian homolog of a Drosophila circadian rhythm gene, in nasopharyngeal carcinoma. Quantitative real-time PCR, western blotting and immunohistochemistry revealed TIMELESS was upregulated in NPC cell lines (n=8 vs. NP69 cells), and freshly-frozen (n=6) and paraffin-embedded human NPC specimens (n=108vs. normal samples/non-tumor cells). TIMELESS expression was associated with T category ( P =0.002), N category ( P =0.001), clinical stage ( P
ISSN:	0304-3835 1872-7980
DOI:	10.1016/j.canlet.2017.05.022