Reproductive effects of life-cycle exposure to difenoconazole on female marine medaka (Oryzias melastigma)

Difenoconazole (DFZ) is a widely used triazole fungicide which has been detected in some estuaries and embayments. This study was conducted to investigate the effects of DFZ on ovarian development in female marine medaka ( Oryzias melastigma ). After 180 days exposure of the embryo to DFZ (0, 1, 10,...

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Veröffentlicht in:Ecotoxicology (London) 2017-08, Vol.26 (6), p.772-781
Hauptverfasser: Dong, Xiaocui, Zuo, Zhenghong, Guo, Jiaojiao, Li, Hongbin, Zhang, Lemeng, Chen, Meng, Yang, Zhibin, Wang, Chonggang
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Sprache:eng
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Zusammenfassung:Difenoconazole (DFZ) is a widely used triazole fungicide which has been detected in some estuaries and embayments. This study was conducted to investigate the effects of DFZ on ovarian development in female marine medaka ( Oryzias melastigma ). After 180 days exposure of the embryo to DFZ (0, 1, 10, 100 and 1000 ng/L), the gonadosomatic index and percentage of mature oocytes produced were significantly reduced in the 1, 10 and 100 ng/L treatments but not the 1000 ng/L treatment compared to the control, thus exhibiting a U-shaped dose response curve. The relative mRNA levels of brain follicle-stimulating hormone, ovarian cytochrome P450 aromatase (CYP19s), hepatic estrogen receptors and vitellogenin, and the ratio of 17β-estradiol to testosterone in the muscle, also showed a U-shaped dose response, which was consistent with the development of oocytes. In addition, glutathione S-transferase activity in the ovary showed a U-shaped dose-response. These results gave an explanation for this U-shaped dose-response. The egg number produced, the hatch ability and the swim-up success in the F1 generation all showed a U-shaped dose response, indicating that exposure to DFZ at low concentrations can cause a decrease of fecundity and viability of the next generation. Thus, a more extensive evaluation of the impact of DFZ on marine fish reproduction at realistic environmental concentrations is needed.
ISSN:0963-9292
1573-3017
DOI:10.1007/s10646-017-1808-1