THE CONTENT OF POLYPHENOLS AND CHOSEN HEAVY METALS IN CHICKPEA SEEDS (CICER ARIETINUM L.) AFTER MICRONUTRIENTS APPLICATION INTO SOIL

The aim of work was the investigation of the input of chosen heavy metals into chickpea and polyphenols accumulation in seeds cultivated after Zn and Cu application into soil in model conditions. The soil used in the pot trial was uncontaminated. Chickpea (cv. Slovák) was harvested in full ripeness. With increased Zn (Cu) doses applied into the soil (0-500 and 0-100 mg.kg-1 for Zn and Cu, respectively), the strong statistical positive relationship between soil Zn (Cu) content and Zn (Cu) amount in seeds of chickpea was confirmed. Despite of high Zn (Cu) doses applied into the soil in model conditions, in all variants the determined Zn and Cu amount in chickpea was below the maximal allowed content in foodstuffs. Chickpea cv. Slovák accumulated high amounts of Pb and Cd (in all variants higher than hygienic limits). The contents of other tested heavy metals (Zn, Cu, Ni, Cr) were lower than hygienic limits. With increased Zn doses applied into the soil the polyphenols contents determined in seeds of chickpea were increased. With increased Cu doses applied into the soil the polyphenols contents determined in seeds of chickpea were decreased. The strong statistical relationship between soil Zn content and polyphenols amount (R=0.911) as well as the antioxidant capacity values (R=0.992) was confirmed. The moderate statistical linear negative relationship between soil Cu content and polyphenols content (R= - 0.671) and increased antioxidant capacity values in seeds of chickpea was confirmed.