Establishment and Application of a Method for Rapid Determination of Total Sugar Content Based on Colorimetric Microplate

To obtain maximal linear regression correlation coefficient ( R 2 ) of glucose standard curve determinations, single-factor test and response surface methodology were applied to optimize conditions including reaction time, incubation temperature and phenol concentration. For investigating optimization of this method, the effects of different metal ions, amino acids and proteins, respectively, were evaluated. The contents of several polysaccharides were determined by three methods, and it was found that the absorption value at 490 nm of the reaction system (230 μL) placed in the microplate involving 150 μL sulfuric acid and 30 μL 5 % phenol solution was in linear relationship with the concentration of glucose range from 10 to 100 nmol/well at 85 °C for 20 min. Our results also suggested that the method had a relative deviation of 0.4 %, and the recovery was between 102.7 and 104.10 %.