Study of DNA interactions with Cu2+ and Mg2+ ions in the presence of caffeine
A study of caffeine effects on the complexation of the DNA molecule with Cu 2+ and Mg 2+ ions in solution is presented. DNA binding with cations and caffeine is traced from changes in DNA circular dichroism and UV absorption spectra. The complexation of caffeine with M 2+ ions is studied by IR spect...
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Veröffentlicht in: | Journal of structural chemistry 2017-03, Vol.58 (2), p.399-405 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A study of caffeine effects on the complexation of the DNA molecule with Cu
2+
and Mg
2+
ions in solution is presented. DNA binding with cations and caffeine is traced from changes in DNA circular dichroism and UV absorption spectra. The complexation of caffeine with M
2+
ions is studied by IR spectroscopy. Caffeine is found to form complexes of different structure with Mg
2+
and Cu
2+
ions in solution. In the presence of caffeine, DNA solutions with MCl
2
show signs of formation of intricate complexes involving nitrogenous bases. In DNA solutions with CuCl
2
, the presence of caffeine causes additional destabilization of the secondary DNA structure. In DNA solutions with MgCl
2
, the presence of caffeine leads to the formation of new complexes with the participation of Mg
2+
, caffeine, and bases without disrupting the secondary DNA structure. |
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ISSN: | 0022-4766 1573-8779 |
DOI: | 10.1134/S0022476617020263 |