Agronomic, Transcriptomic and Metabolomic Expression Analysis of Aroma Gene (badh2) under Different Temperature Regimes in Rice

The effects of environmental factors such as salinity, water, aging, heat, cold and shading on 2AP concentration or badh2 gene expression or agronomic traits were investigated individually but the effects of the optimum temperature (20°C to 30°C) were not studied extensively. [...]this study investigated the consequences of three different temperatures (ambient or 28.29 ñ 0.91°C, 25°C and 20°C) on agronomic performance, badh2 gene expression, 2AP concentration and phenotypic aroma score of five aromatic and a non-aromatic (control) rice genotype. The agronomic performance such as the flowering days (121.60), days to maturity (151.20), plant height (119.80 cm), panicle length (34.80 cm), fertile grain per panicle (151.40), 1000 grain weight (32.67 g) and grain yield per plant (76.73 g) were higher at 25°C temperature compared to the ambient and 20°C temperature. [...]the badh2 gene expression level and 2AP concentration were influenced by the temperature, which also regulated the phenotypic expression of aroma and agronomic performance of aromatic rice. (2008) mentioned that homozygous recessive allele of badh2 gene also induced the formation of 2AP and rice becomes aromatic. [...]gene expression analysis of badh2 gene using reverse transcription quantitative PCR (RTqPCR) with relative quantification method could explore the genetic condition of aromatic rice as well as the influences of temperature on aroma gene by analyzing biochemical and physiological changes of aromatic rice due to the changes of badh2 gene expression level (Livak and Schmittgen, 2001; Kim et al., 2003). (2000) reported that high humidity (70 to 80%) within a temperature range of 25°C to...