FRI0053 Correlation Between Substance P Serum Levels and Disease Activity in Rheumatoid Arthritis

BackgroundSubstance P (SP) is a neuropeptide involved in the neurogenic inflammation of rheumatoid arthritis (RA) and is increased in serum and synovial fluid of patients with the disease.ObjectivesTo evaluate the possible correlation between SP serum levels and RA activity assessed by disease activity score (DAS) 28 joints-C reactive protein (CRP) and German 7-Joint Ultrasound Score (GUS7).MethodsNinety RA patients (32 in remission, 8 with low disease activity, 42 with moderate disease activity and 8 with high disease activity assessed by DAS28-PCR) and 24 healthy controls were included. GUS7 was performed by evaluating synovitis and synovial/tenosynovial vascularity semiquantitatively (grade 0–3) by gray-scale (GS) and power Doppler (PD) ultrasound using an 18-mHz linear transducer. SP serum levels were measured by enzyme-linked immunosorbent assay. Correlation was estimated by Spearman's correlation coefficient.ResultsSP serum levels were statistically different between controls and patients in remission or with different grades of disease activity (P≤0.05). Furthermore, positive tendency between SP serum levels and RA activity was observed. SP showed significant correlation with DAS28-CRP, tender joint count, swollen joint count and visual analog scale (VAS) by Spearman's correlation coefficient. No correlation was found between SP and CRP or GUS7.ConclusionsSP appears to be a potential RA activity biomarker. Notwithstanding, it is unlikely that SP itself provides sufficient discrimination in regard to rheumatoid arthritis activity given the complexity and heterogeneous etiology of this disease. In this regard, further studies of SP as a biological marker are necessary for its inclusion in a multi-biomarker equation that considers the neurogenic pathway participation in the pathogenesis of RA.ReferencesO'Connor TM, O'Connell J, O'Brien DI, Goode T, Bredin CP, Shanahan F. The Role of Substance P in Inflammatory Disease. J Cell Physiol 2004; 201:167-180.Hernanz A, De Miguel E, Romera N, Perez-Ayala C, Gijon J, Arnalich F. Calcitonin gene-related peptide II, substance P and vasoactive intestinal peptide in plasma and synovial fluid from patients with inflammatory joint disease. Br J Rheumatol 1993; 32:31-35.Grimsholm O, Rantapää-Dahlqvist S, Forsgren S. Levels of gastrin-releasing peptide and substance P in synovial fluid and serum correlate with levels of cytokines in rheumatoid arthritis. Arthritis Res Ther 2005; 7:R416-R426.Origuchi T, Iwamoto N, Kawas