Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles
To reveal the sensing of tetrodotoxin (TTX) by tiger puffer Takifugu rubripes juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-...
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Veröffentlicht in: | Fisheries science 2017-05, Vol.83 (3), p.401-412 |
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creator | Okita, Kogen Tan, Engkong Satone, Hina Kinoshita, Shigeharu Asakawa, Shuichi Ojima, Daisuke Yamazaki, Hideki Sakiyama, Kazutaka Takatani, Tomohiro Arakawa, Osamu Hagiwara, Atsushi Sakakura, Yoshitaka |
description | To reveal the sensing of tetrodotoxin (TTX) by tiger puffer
Takifugu rubripes
juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the
T. rubripes
genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of
T. rubripes
juveniles is controlled in the feeding center in the brain, that
T. rubripes
may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion. |
doi_str_mv | 10.1007/s12562-017-1079-z |
format | Article |
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Takifugu rubripes
juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the
T. rubripes
genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of
T. rubripes
juveniles is controlled in the feeding center in the brain, that
T. rubripes
may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.</description><identifier>ISSN: 0919-9268</identifier><identifier>EISSN: 1444-2906</identifier><identifier>DOI: 10.1007/s12562-017-1079-z</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Biomedical and Life Sciences ; Brain ; Central nervous system ; Detection ; Epithelium ; Feeding ; Fish & Wildlife Biology & Management ; Fish culture ; Fish skin ; Fisheries ; Food Science ; Freshwater & Marine Ecology ; Gene expression ; Gene mapping ; Gene sequencing ; Genes ; Ingestion ; Juveniles ; Life Sciences ; Mapping ; Minors ; Nervous system ; Nucleotides ; Odour ; Olfaction ; Olfactory epithelium ; Olfactory system ; Original Article ; Reinforcement ; Sampling ; Smell ; Takifugu rubripes ; Tetrodotoxin ; Toxins</subject><ispartof>Fisheries science, 2017-05, Vol.83 (3), p.401-412</ispartof><rights>Japanese Society of Fisheries Science 2017</rights><rights>Fisheries Science is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c453t-e05209a54ab5bfd21b0c852cb1d6b9fe836ab077ac62d2455906f54c63aff7393</citedby><cites>FETCH-LOGICAL-c453t-e05209a54ab5bfd21b0c852cb1d6b9fe836ab077ac62d2455906f54c63aff7393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12562-017-1079-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12562-017-1079-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Okita, Kogen</creatorcontrib><creatorcontrib>Tan, Engkong</creatorcontrib><creatorcontrib>Satone, Hina</creatorcontrib><creatorcontrib>Kinoshita, Shigeharu</creatorcontrib><creatorcontrib>Asakawa, Shuichi</creatorcontrib><creatorcontrib>Ojima, Daisuke</creatorcontrib><creatorcontrib>Yamazaki, Hideki</creatorcontrib><creatorcontrib>Sakiyama, Kazutaka</creatorcontrib><creatorcontrib>Takatani, Tomohiro</creatorcontrib><creatorcontrib>Arakawa, Osamu</creatorcontrib><creatorcontrib>Hagiwara, Atsushi</creatorcontrib><creatorcontrib>Sakakura, Yoshitaka</creatorcontrib><title>Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles</title><title>Fisheries science</title><addtitle>Fish Sci</addtitle><description>To reveal the sensing of tetrodotoxin (TTX) by tiger puffer
Takifugu rubripes
juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the
T. rubripes
genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of
T. rubripes
juveniles is controlled in the feeding center in the brain, that
T. rubripes
may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.</description><subject>Biomedical and Life Sciences</subject><subject>Brain</subject><subject>Central nervous system</subject><subject>Detection</subject><subject>Epithelium</subject><subject>Feeding</subject><subject>Fish & Wildlife Biology & Management</subject><subject>Fish culture</subject><subject>Fish skin</subject><subject>Fisheries</subject><subject>Food Science</subject><subject>Freshwater & Marine Ecology</subject><subject>Gene expression</subject><subject>Gene mapping</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Ingestion</subject><subject>Juveniles</subject><subject>Life Sciences</subject><subject>Mapping</subject><subject>Minors</subject><subject>Nervous system</subject><subject>Nucleotides</subject><subject>Odour</subject><subject>Olfaction</subject><subject>Olfactory epithelium</subject><subject>Olfactory system</subject><subject>Original Article</subject><subject>Reinforcement</subject><subject>Sampling</subject><subject>Smell</subject><subject>Takifugu 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analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles</title><author>Okita, Kogen ; Tan, Engkong ; Satone, Hina ; Kinoshita, Shigeharu ; Asakawa, Shuichi ; Ojima, Daisuke ; Yamazaki, Hideki ; Sakiyama, Kazutaka ; Takatani, Tomohiro ; Arakawa, Osamu ; Hagiwara, Atsushi ; Sakakura, Yoshitaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-e05209a54ab5bfd21b0c852cb1d6b9fe836ab077ac62d2455906f54c63aff7393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Biomedical and Life Sciences</topic><topic>Brain</topic><topic>Central nervous system</topic><topic>Detection</topic><topic>Epithelium</topic><topic>Feeding</topic><topic>Fish & Wildlife Biology & Management</topic><topic>Fish culture</topic><topic>Fish skin</topic><topic>Fisheries</topic><topic>Food Science</topic><topic>Freshwater & Marine Ecology</topic><topic>Gene expression</topic><topic>Gene mapping</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Ingestion</topic><topic>Juveniles</topic><topic>Life Sciences</topic><topic>Mapping</topic><topic>Minors</topic><topic>Nervous system</topic><topic>Nucleotides</topic><topic>Odour</topic><topic>Olfaction</topic><topic>Olfactory epithelium</topic><topic>Olfactory system</topic><topic>Original Article</topic><topic>Reinforcement</topic><topic>Sampling</topic><topic>Smell</topic><topic>Takifugu rubripes</topic><topic>Tetrodotoxin</topic><topic>Toxins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okita, Kogen</creatorcontrib><creatorcontrib>Tan, Engkong</creatorcontrib><creatorcontrib>Satone, Hina</creatorcontrib><creatorcontrib>Kinoshita, Shigeharu</creatorcontrib><creatorcontrib>Asakawa, Shuichi</creatorcontrib><creatorcontrib>Ojima, Daisuke</creatorcontrib><creatorcontrib>Yamazaki, Hideki</creatorcontrib><creatorcontrib>Sakiyama, Kazutaka</creatorcontrib><creatorcontrib>Takatani, Tomohiro</creatorcontrib><creatorcontrib>Arakawa, Osamu</creatorcontrib><creatorcontrib>Hagiwara, Atsushi</creatorcontrib><creatorcontrib>Sakakura, Yoshitaka</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Research Library (Alumni 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Sci</stitle><date>2017-05-01</date><risdate>2017</risdate><volume>83</volume><issue>3</issue><spage>401</spage><epage>412</epage><pages>401-412</pages><issn>0919-9268</issn><eissn>1444-2906</eissn><abstract>To reveal the sensing of tetrodotoxin (TTX) by tiger puffer
Takifugu rubripes
juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the
T. rubripes
genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of
T. rubripes
juveniles is controlled in the feeding center in the brain, that
T. rubripes
may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><doi>10.1007/s12562-017-1079-z</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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source | Springer Nature - Complete Springer Journals |
subjects | Biomedical and Life Sciences Brain Central nervous system Detection Epithelium Feeding Fish & Wildlife Biology & Management Fish culture Fish skin Fisheries Food Science Freshwater & Marine Ecology Gene expression Gene mapping Gene sequencing Genes Ingestion Juveniles Life Sciences Mapping Minors Nervous system Nucleotides Odour Olfaction Olfactory epithelium Olfactory system Original Article Reinforcement Sampling Smell Takifugu rubripes Tetrodotoxin Toxins |
title | Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles |
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