Conversion of raw glycerol to microbial lipids by new Metschnikowia and Yarrowia lipolytica strains
Nine Metschnikowia spp. strains and six Yarrowia lipolytica strains were tested for lipid production on raw glycerol from a biodiesel plant. Metschnikowia sp. 271 and Y. lipolytica 347 were selected for biomass and lipid production, and optimized using central composite factorial design (CCD) and re...
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Veröffentlicht in: | Annals of microbiology 2016-12, Vol.66 (4), p.1409-1418 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Nine
Metschnikowia
spp. strains and six
Yarrowia lipolytica
strains were tested for lipid production on raw glycerol from a biodiesel plant.
Metschnikowia
sp. 271 and
Y. lipolytica
347 were selected for biomass and lipid production, and optimized using central composite factorial design (CCD) and response surface methodology (RSM). The lipid production (2.60 g/L) of
Y. lipolytica
347 was maximized at C/N 118, time 144 h and 90 g/L glycerol, while the maximum lipid production (0.49 g/L) of
Metschnikowia
sp. 271 was obtained under similar conditions but after a further incubation for 7 days at 16 °C. The fatty acids profile of
Y. lipolytica
exhibited a considerable amount of C18:1n7 (~36 %), C18:1n9 (~16 %), and C16:0 (~16 %), whereas
Metschnikowia
sp. produced mainly C18:1n9 (~33 %), C16:0 (~21 %), and C16:1n7 (~21 %). Both yeasts showed similar amounts of unsaturated fatty acids (~70 %). However, considerable amounts of polyunsaturated fatty acid (PUFAs) were exhibited only by
Metschnikowia
sp. (~12 %). |
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ISSN: | 1590-4261 1869-2044 |
DOI: | 10.1007/s13213-016-1228-0 |