Synthesis and anticancer activity of novel 2-substituted pyranopyridine derivatives

A novel series of 2-aminopyranopyridine derivatives ( 3 – 19 ) were synthesized utilizing 2-chloro-4-(thiophen-2-yl)-7,8-dihydro-5 H -pyrano[4,3- b ]pyridine-3-carbonitrile ( 2 ) as a key starting compound. The structures of the newly synthesized compounds were confirmed on the basis of elemental analyses and infrared (IR), 1 H, 13 C-nuclear magnetic resonance (NMR) and mass spectra. Anticancer evaluation was carried out for the new derivatives against Hep-G2 (human liver carcinoma), MCF-7 (human breast carcinoma), Caco-2 (human colorectal adenocarcinoma) and HCT116 (human colorectal carcinoma) cell lines using doxorubicin as a reference drug. The biological results revealed that the compounds 12 and 14 exhibited more potent anticancer activity than the reference drug.