Epac‐Rap1‐activated mesenchymal stem cells improve cardiac function in rat model of myocardial infarction

Summary Introduction Rap1, a member of Ras superfamily of small GTP‐binding proteins, is involved in cardiovascular biology in numerous ways. It is an evolutionary conserved regulator of adhesion, polarity, differentiation and growth. Aims Our aim was to analyze Rap1‐activated rat bone marrow mesenc...

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Veröffentlicht in:Cardiovascular therapeutics 2017-04, Vol.35 (2), p.n/a
Hauptverfasser: Khan, Irfan, Ali, Anwar, Akhter, Muhammad Aleem, Naeem, Nadia, Chotani, Maqsood Ahmed, Iqbal, Hana'a, Kabir, Nurul, Atiq, Mehnaz, Salim, Asmat
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Sprache:eng
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Zusammenfassung:Summary Introduction Rap1, a member of Ras superfamily of small GTP‐binding proteins, is involved in cardiovascular biology in numerous ways. It is an evolutionary conserved regulator of adhesion, polarity, differentiation and growth. Aims Our aim was to analyze Rap1‐activated rat bone marrow mesenchymal stem cells (MSCs) for their potential role in adhesion and cardiac differentiation. Methods Myocardial infarction (MI) was produced in Sprague Dawley (SD) rats through occlusion of the left anterior descending coronary artery. MSCs were treated with 8‐pCPT‐2′‐O‐Me‐cAMP (CPT) to activate Rap1. Normal (untreated) and CPT‐treated MSCs were transplanted through intramyocardial injection in respective groups. Cardiac function was assessed by echocardiography at 2 and 4 weeks after cell transplantation. Histological analysis was performed to observe changes at tissue level. Results Homing of CPT‐treated MSCs was significantly (***P
ISSN:1755-5914
1755-5922
DOI:10.1111/1755-5922.12248