Immuno-PCR technology for detection of natural human antibodies against Le^sup c^ disaccharide

Immuno-PCR technology for detection of natural human antibodies against Le^sup c^ disaccharide

The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-LeC IgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to th...

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Veröffentlicht in:Glycoconjugate journal 2017-04, Vol.34 (2), p.199
Hauptverfasser: Maerle, Artem V, Voronina, Daria V, Dobrochaeva, Kira L, Galanina, Oxana E, Alekseev, Leonid P, Bovin, Nicolai V, Zavriev, Sergei K, Ryazantsev, Dmitriy Yu
Format: Artikel
Sprache:eng
Schlagworte:
Antigens
Bioassays
Immune system
Immunoglobulins
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Zusammenfassung:The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-LeC IgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to the conventional ELISA with the same antigen.
ISSN:0282-0080
1573-4986
DOI:10.1007/s10719-016-9751-6