SULFATED PACHYMARAN PROTECTS PC12 CELLS AGAINST MPP+-INDUCED INJURY

SULFATED PACHYMARAN PROTECTS PC12 CELLS AGAINST MPP+-INDUCED INJURY

PC12 cells treated with 0.5 mM MPP^sup +^ for 24 h were used as PD model in vitro to evaluate the neuroprotective effects of sulfated pachymaran (SP). Cell viability and apoptosis were detected by MTT assay, inverted microscope and Hoechst 33342 staining, respectively. Expression levels of Bcl-2, Ba...

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Veröffentlicht in:Current topics in nutraceuticals research 2016-05, Vol.14 (2), p.127
Hauptverfasser: Gao, Gui-Zhen, Wu, Chao, Xue, Hong-Yu, Shan, Ling-Ling, Meng, Qing-Zhen
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
Cytochrome
Membranes
Parkinson's disease
Proteins
Science
Software
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Zusammenfassung:PC12 cells treated with 0.5 mM MPP^sup +^ for 24 h were used as PD model in vitro to evaluate the neuroprotective effects of sulfated pachymaran (SP). Cell viability and apoptosis were detected by MTT assay, inverted microscope and Hoechst 33342 staining, respectively. Expression levels of Bcl-2, Bax and caspase-3 were detected by Western blotting. SP at the concentrations from 0.5 to 4 mg/mL is safe to PC12 cells. MPP+ treatment significantly decreased the cell viability, the expressions of Bcl-2 and Caspase-3 (P
ISSN:1540-7535
2641-452X