Rheological properties of gluten derived from wheat cultivars with identical HMW glutenin subunit composition

Ten spring wheat cultivars possessing identical HMW glutenin subunits (2*, 7+8, 5+10) were evaluated for gluten and protein content. Gluten content was related to flour protein content (r=0·98). Addition of freeze‐dried gluten to the base flour (cv Alpha) to a constant protein level of 12% generally increased dough strength. However, the magnitude of variation in mixing patterns depended more on the type of the supplemental gluten. Fortification of the base flour with the freeze‐dried gluten from the cv Glenlea produced mixographs with the longest mixing development time (MDT), and highest band width energy (BWE) and energy to peak (ETP), suggesting that the source of gluten had a strong effect on dough rheology. The viscoelastic properties of undiluted wet gluten varied between cultivars during mixing reflecting differences in gluten quality. Freshly prepared wet gluten of Glenlea showed extended mixing tolerance as compared to Norseman or Alpha gluten. The wet gluten from cv Glenlea was less extensible with high maximum resistance to extension and had a larger area under the extensigraph curve than gluten obtained from cv Norseman. Gluten prepared from the cultivars Glenlea, Bluesky and Wildcat were less soluble in aqueous propanol and produced more froth when the dough was washed with deionised water. The froth proteins, separated by SDS‐PAGE, predominantly contained strongly stained bands in the region corresponding to molecular weight