Comparison of the Precipitation of Alfalfa Leaf Protein and Bovine Serum Albumin by Tannins in the Radial Diffusion Method

Comparison of the Precipitation of Alfalfa Leaf Protein and Bovine Serum Albumin by Tannins in the Radial Diffusion Method

The precipitation of protein by condensed and hydrolysable tannins was evaluated with the radial diffusion method of Hagerman (1987) using bovine serum albumin (BSA) and isolated leaf protein from fresh alfalfa (Medicago sativa). Alfalfa leaf protein (AALP) was included at two concentrations, 25 and...

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Veröffentlicht in:Journal of the science of food and agriculture 1997-08, Vol.74 (4), p.513-523
Hauptverfasser: Giner-Chavez, B I, Van Soest, P J, Robertson, J B, Lascano, C, Pell, A N
Format: Artikel
Sprache:eng
Schlagworte:
alfalfa leaf protein
Biological and medical sciences
bovine serum albumin
condensed tannins
Feed and pet food industries
Food industries
Fundamental and applied biological sciences. Psychology
protein precipitation
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Zusammenfassung:The precipitation of protein by condensed and hydrolysable tannins was evaluated with the radial diffusion method of Hagerman (1987) using bovine serum albumin (BSA) and isolated leaf protein from fresh alfalfa (Medicago sativa). Alfalfa leaf protein (AALP) was included at two concentrations, 25 and 156 mg N litre‐1, at pH 6·8 and 39°C to simulate rumen conditions. The condensed tannins were purified from lyophilised samples of Arachis pintoi, Desmodium ovalifolium, Gliricidia sepium, Manihot esculenta and quebracho (Schinopsis balansae). Hydrolysable tannins from tannic acid (TA) were used as well. There was a significant interaction (P
ISSN:0022-5142
1097-0010
DOI:10.1002/(SICI)1097-0010(199708)74:4<513::AID-JSFA825>3.0.CO;2-B