Prévalence et potentiel de virulence in vitro de Aeromonas sp. chez la grenouille comestible Hoplobatrachus occipitalis (Ranidae) collectée dans le Centre Ouest de la Côte d'Ivoire/Prevalence and in vitro potential virulence of Aeromonas sp. from edible frog Hoplobatrachus occipitalis (Ranidae) collected in Middle West of Côte d'Ivoire

Edible frogs Hoplobatrachus occipitalis represent an animal protein source for most population of West area of Côte d'Ivoire, whose sanitary quality are understand. The objectives of this study was to determinate the rate of Aeromonas sp. from these frogs and to evaluate in vitro potential virulence of the strains. A total of 300 frog including 210 fresh and 90 smoked from three markets were collected. The analyses of samples consisted to isolation of Aeromonas, the biotypage and the determination of in vitro virulence factors. The results reveal that no smoked frog is contaminated; on other hand, the percentage of contamination of fresh frog is 23.0% with the prevalence of 70.5%. Species Aeromonas hydrophila (76.4 %) and Aeromonas sobria (23.6%) have been exclusively identified. The biotype of Aeromonas hydrophila characterized by no utilization of mannose and citrate was most frequent (41.7%). The determinants of the virulence of strains are the production of hemolysin (70.1%), protease (68.9%), ADNase (97.3%), lipase (41.1%) and amylase (55.4%). The high prevalence of Aeromonas with a potential pathogen from edible frog, represent a risk for consumers and require the measure of assurance for the sanitary safety of products.