Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender
Cryopreservation is considered that it decreases sperm quality about 50 percent therefore research is being conducted to increase the frozen-thawed quality of bull sperm. The object of study was to examine the effect of polyunsaturated fatty acids on the frozen-thawed bull semen sperm characteristic...
Gespeichert in:
| Veröffentlicht in: | Pure and applied biology 2016-09, Vol.5 (3), p.399 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | 399 |
| container_title | Pure and applied biology |
| container_volume | 5 |
| creator | Kaka, Asmatullah |
| description | Cryopreservation is considered that it decreases sperm quality about 50 percent therefore research is being conducted to increase the frozen-thawed quality of bull sperm. The object of study was to examine the effect of polyunsaturated fatty acids on the frozen-thawed bull semen sperm characteristics. Semen was collected from three bulls with an average of 8 collection from each bull at interval of 3 days. Semen ejaculates with motility and normal morphology 70% and 80% respectively were processed in extender (BioXcell®) having combined supplementation 0 to 15ng/ml of alpha linolenic acid (ALA) and docosohexaenoic acid (DHA). Extended sample was cooled at 5°C for 2 hours, packaged and frozen into straws and in liquid nitrogen for 24 hours. Sequentially, straws were examined for sperm cytological characteristics, lipid peroxidation, fatty acid concentration and super oxide dismutase (SOD) after thawing. Sperm cytological characteristics decreased in the supplemented groups in comparison to control group. Docosahexaenoic acid improved in all supplemented groups, whereas ALA improved at 5ng/ml of ALA. Superoxide dismutase and lipid peroxidation were improved linearly by increasing fatty acids. Conclusion, combination of ALA and DHA has detrimental effect on frozen-thawed bull sperm parameters. |
| doi_str_mv | 10.19045/bspab.2016.50052 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_1814077690</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4159468481</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1132-6e5a6d68da0c3c8385ca221bdb03ef54020c37de3fcbfe49aac20aa3cc54f4923</originalsourceid><addsrcrecordid>eNpNkEtOwzAQhiMEEhX0AOwssU7xM48lVOUhVWID62jijKmrNA62A5QLcBsOwclIWxbMZkbzf5qRviS5YHTGSirVVR16qGecsmymKFX8KJlwQWXKZV4c_5tPk2kIazpWmQuaq0nytTAGdSTOENulbzZ6R8LQ9y1usIsQret2We_a7dAFiIOHiA0xEOOWgLZNICNhvPvELo0reB_DemhbEnr0G6JX4EFH9DZEqwMZgu1eyI11Hxrb9ueb4EfErkF_npwYaANO__pZ8ny7eJrfp8vHu4f59TLVjAmeZqgga7KiAaqFLkShNHDO6qamAo2SlI_7vEFhdG1QlgCaUwChtZJGllycJZeHu713rwOGWK3d4LvxZcUKJmmeZyUdKXagtHcheDRV7-0G_LZitNorr_bKq53yaq9c_AJ4RXoG</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1814077690</pqid></control><display><type>article</type><title>Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender</title><source>EZB Electronic Journals Library</source><creator>Kaka, Asmatullah</creator><creatorcontrib>Kaka, Asmatullah</creatorcontrib><description>Cryopreservation is considered that it decreases sperm quality about 50 percent therefore research is being conducted to increase the frozen-thawed quality of bull sperm. The object of study was to examine the effect of polyunsaturated fatty acids on the frozen-thawed bull semen sperm characteristics. Semen was collected from three bulls with an average of 8 collection from each bull at interval of 3 days. Semen ejaculates with motility and normal morphology 70% and 80% respectively were processed in extender (BioXcell®) having combined supplementation 0 to 15ng/ml of alpha linolenic acid (ALA) and docosohexaenoic acid (DHA). Extended sample was cooled at 5°C for 2 hours, packaged and frozen into straws and in liquid nitrogen for 24 hours. Sequentially, straws were examined for sperm cytological characteristics, lipid peroxidation, fatty acid concentration and super oxide dismutase (SOD) after thawing. Sperm cytological characteristics decreased in the supplemented groups in comparison to control group. Docosahexaenoic acid improved in all supplemented groups, whereas ALA improved at 5ng/ml of ALA. Superoxide dismutase and lipid peroxidation were improved linearly by increasing fatty acids. Conclusion, combination of ALA and DHA has detrimental effect on frozen-thawed bull sperm parameters.</description><identifier>ISSN: 2304-2478</identifier><identifier>EISSN: 2304-2478</identifier><identifier>DOI: 10.19045/bspab.2016.50052</identifier><language>eng</language><publisher>Quetta: International Society of Pure and Applied Biology</publisher><subject>Artificial insemination ; Fatty acids ; Fertility ; Fish oils ; Lipid peroxidation ; Lipids ; Morphology ; Motility ; Oxidation ; Peroxidation ; Polyunsaturated fatty acids ; Quality ; Sperm ; Thawing</subject><ispartof>Pure and applied biology, 2016-09, Vol.5 (3), p.399</ispartof><rights>Copyright International Society of Pure and Applied Biology Sep 2016</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Kaka, Asmatullah</creatorcontrib><title>Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender</title><title>Pure and applied biology</title><description>Cryopreservation is considered that it decreases sperm quality about 50 percent therefore research is being conducted to increase the frozen-thawed quality of bull sperm. The object of study was to examine the effect of polyunsaturated fatty acids on the frozen-thawed bull semen sperm characteristics. Semen was collected from three bulls with an average of 8 collection from each bull at interval of 3 days. Semen ejaculates with motility and normal morphology 70% and 80% respectively were processed in extender (BioXcell®) having combined supplementation 0 to 15ng/ml of alpha linolenic acid (ALA) and docosohexaenoic acid (DHA). Extended sample was cooled at 5°C for 2 hours, packaged and frozen into straws and in liquid nitrogen for 24 hours. Sequentially, straws were examined for sperm cytological characteristics, lipid peroxidation, fatty acid concentration and super oxide dismutase (SOD) after thawing. Sperm cytological characteristics decreased in the supplemented groups in comparison to control group. Docosahexaenoic acid improved in all supplemented groups, whereas ALA improved at 5ng/ml of ALA. Superoxide dismutase and lipid peroxidation were improved linearly by increasing fatty acids. Conclusion, combination of ALA and DHA has detrimental effect on frozen-thawed bull sperm parameters.</description><subject>Artificial insemination</subject><subject>Fatty acids</subject><subject>Fertility</subject><subject>Fish oils</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Morphology</subject><subject>Motility</subject><subject>Oxidation</subject><subject>Peroxidation</subject><subject>Polyunsaturated fatty acids</subject><subject>Quality</subject><subject>Sperm</subject><subject>Thawing</subject><issn>2304-2478</issn><issn>2304-2478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpNkEtOwzAQhiMEEhX0AOwssU7xM48lVOUhVWID62jijKmrNA62A5QLcBsOwclIWxbMZkbzf5qRviS5YHTGSirVVR16qGecsmymKFX8KJlwQWXKZV4c_5tPk2kIazpWmQuaq0nytTAGdSTOENulbzZ6R8LQ9y1usIsQret2We_a7dAFiIOHiA0xEOOWgLZNICNhvPvELo0reB_DemhbEnr0G6JX4EFH9DZEqwMZgu1eyI11Hxrb9ueb4EfErkF_npwYaANO__pZ8ny7eJrfp8vHu4f59TLVjAmeZqgga7KiAaqFLkShNHDO6qamAo2SlI_7vEFhdG1QlgCaUwChtZJGllycJZeHu713rwOGWK3d4LvxZcUKJmmeZyUdKXagtHcheDRV7-0G_LZitNorr_bKq53yaq9c_AJ4RXoG</recordid><startdate>20160910</startdate><enddate>20160910</enddate><creator>Kaka, Asmatullah</creator><general>International Society of Pure and Applied Biology</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T7</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20160910</creationdate><title>Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender</title><author>Kaka, Asmatullah</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1132-6e5a6d68da0c3c8385ca221bdb03ef54020c37de3fcbfe49aac20aa3cc54f4923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Artificial insemination</topic><topic>Fatty acids</topic><topic>Fertility</topic><topic>Fish oils</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Morphology</topic><topic>Motility</topic><topic>Oxidation</topic><topic>Peroxidation</topic><topic>Polyunsaturated fatty acids</topic><topic>Quality</topic><topic>Sperm</topic><topic>Thawing</topic><toplevel>online_resources</toplevel><creatorcontrib>Kaka, Asmatullah</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ProQuest research library</collection><collection>ProQuest Biological Science Journals</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Pure and applied biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaka, Asmatullah</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender</atitle><jtitle>Pure and applied biology</jtitle><date>2016-09-10</date><risdate>2016</risdate><volume>5</volume><issue>3</issue><spage>399</spage><pages>399-</pages><issn>2304-2478</issn><eissn>2304-2478</eissn><abstract>Cryopreservation is considered that it decreases sperm quality about 50 percent therefore research is being conducted to increase the frozen-thawed quality of bull sperm. The object of study was to examine the effect of polyunsaturated fatty acids on the frozen-thawed bull semen sperm characteristics. Semen was collected from three bulls with an average of 8 collection from each bull at interval of 3 days. Semen ejaculates with motility and normal morphology 70% and 80% respectively were processed in extender (BioXcell®) having combined supplementation 0 to 15ng/ml of alpha linolenic acid (ALA) and docosohexaenoic acid (DHA). Extended sample was cooled at 5°C for 2 hours, packaged and frozen into straws and in liquid nitrogen for 24 hours. Sequentially, straws were examined for sperm cytological characteristics, lipid peroxidation, fatty acid concentration and super oxide dismutase (SOD) after thawing. Sperm cytological characteristics decreased in the supplemented groups in comparison to control group. Docosahexaenoic acid improved in all supplemented groups, whereas ALA improved at 5ng/ml of ALA. Superoxide dismutase and lipid peroxidation were improved linearly by increasing fatty acids. Conclusion, combination of ALA and DHA has detrimental effect on frozen-thawed bull sperm parameters.</abstract><cop>Quetta</cop><pub>International Society of Pure and Applied Biology</pub><doi>10.19045/bspab.2016.50052</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2304-2478 |
| ispartof | Pure and applied biology, 2016-09, Vol.5 (3), p.399 |
| issn | 2304-2478 2304-2478 |
| language | eng |
| recordid | cdi_proquest_journals_1814077690 |
| source | EZB Electronic Journals Library |
| subjects | Artificial insemination Fatty acids Fertility Fish oils Lipid peroxidation Lipids Morphology Motility Oxidation Peroxidation Polyunsaturated fatty acids Quality Sperm Thawing |
| title | Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A24%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20in-vitro%20supplementation%20of%20polyunsaturated%20fatty%20acids%20on%20frozen-thawed%20bull%20sperm%20characteristics%20using%20Bioxcell%C2%AE%20extender&rft.jtitle=Pure%20and%20applied%20biology&rft.au=Kaka,%20Asmatullah&rft.date=2016-09-10&rft.volume=5&rft.issue=3&rft.spage=399&rft.pages=399-&rft.issn=2304-2478&rft.eissn=2304-2478&rft_id=info:doi/10.19045/bspab.2016.50052&rft_dat=%3Cproquest_cross%3E4159468481%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1814077690&rft_id=info:pmid/&rfr_iscdi=true |