Effect of in-vitro supplementation of polyunsaturated fatty acids on frozen-thawed bull sperm characteristics using Bioxcell® extender

Cryopreservation is considered that it decreases sperm quality about 50 percent therefore research is being conducted to increase the frozen-thawed quality of bull sperm. The object of study was to examine the effect of polyunsaturated fatty acids on the frozen-thawed bull semen sperm characteristics. Semen was collected from three bulls with an average of 8 collection from each bull at interval of 3 days. Semen ejaculates with motility and normal morphology 70% and 80% respectively were processed in extender (BioXcell®) having combined supplementation 0 to 15ng/ml of alpha linolenic acid (ALA) and docosohexaenoic acid (DHA). Extended sample was cooled at 5°C for 2 hours, packaged and frozen into straws and in liquid nitrogen for 24 hours. Sequentially, straws were examined for sperm cytological characteristics, lipid peroxidation, fatty acid concentration and super oxide dismutase (SOD) after thawing. Sperm cytological characteristics decreased in the supplemented groups in comparison to control group. Docosahexaenoic acid improved in all supplemented groups, whereas ALA improved at 5ng/ml of ALA. Superoxide dismutase and lipid peroxidation were improved linearly by increasing fatty acids. Conclusion, combination of ALA and DHA has detrimental effect on frozen-thawed bull sperm parameters.