Detection and isolation of Bluetongue virus from commercial vaccine batches
Highlights • BTV contamination was identified in Sheep pox and Lumpy skin disease vaccines using real-time PCR. • Infective BTV from the Sheep pox vaccine was isolated and cultured in chicken eggs and Vero cells. • The contaminating virus identity was confirmed as BTV by sequencing.
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| Veröffentlicht in: | Vaccine 2016-06, Vol.34 (28), p.3317-3323 |
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| creator | Bumbarov, Velizar Golender, Natalia Erster, Oran Khinich, Yevgeny |
| description | Highlights • BTV contamination was identified in Sheep pox and Lumpy skin disease vaccines using real-time PCR. • Infective BTV from the Sheep pox vaccine was isolated and cultured in chicken eggs and Vero cells. • The contaminating virus identity was confirmed as BTV by sequencing. |
| doi_str_mv | 10.1016/j.vaccine.2016.03.097 |
| format | Article |
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| subjects | Allergy and Immunology Animals Bluetongue virus Bluetongue virus - isolation & purification Cercopithecus aethiops Chick Embryo Drug Contamination Genes, Viral Manufacturers NS3 Pathogens Real-time PCR Real-Time Polymerase Chain Reaction Skin diseases Vaccine safety Vaccines Vero Cells Viral Vaccines - analysis Viruses VP1 |
| title | Detection and isolation of Bluetongue virus from commercial vaccine batches |
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