Detection and isolation of Bluetongue virus from commercial vaccine batches

Highlights • BTV contamination was identified in Sheep pox and Lumpy skin disease vaccines using real-time PCR. • Infective BTV from the Sheep pox vaccine was isolated and cultured in chicken eggs and Vero cells. • The contaminating virus identity was confirmed as BTV by sequencing.

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Bibliographische Detailangaben
Veröffentlicht in:	Vaccine 2016-06, Vol.34 (28), p.3317-3323
Hauptverfasser:	Bumbarov, Velizar, Golender, Natalia, Erster, Oran, Khinich, Yevgeny
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Bluetongue virus Bluetongue virus - isolation & purification Cercopithecus aethiops Chick Embryo Drug Contamination Genes, Viral Manufacturers NS3 Pathogens Real-time PCR Real-Time Polymerase Chain Reaction Skin diseases Vaccine safety Vaccines Vero Cells Viral Vaccines - analysis Viruses VP1
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Beschreibung
Zusammenfassung:	Highlights • BTV contamination was identified in Sheep pox and Lumpy skin disease vaccines using real-time PCR. • Infective BTV from the Sheep pox vaccine was isolated and cultured in chicken eggs and Vero cells. • The contaminating virus identity was confirmed as BTV by sequencing.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2016.03.097