Effect of vitamin D^sub 3^, zilpaterol hydrochloride supplementation, and postmortem aging on shear force measurements of three muscles in finishing beef steers 1,2

Vitamin D3 (D3) supplementation may be used to increase tenderness in beef from cattle fed zilpaterol hydrochloride (ZH). The study was arranged as a 2 ... 2 factorial with fixed effects of ZH (no ZH or ZH fed at 8.3 mg/kg DM for 20 d with a 3-d withdrawal) and D3 (no D3 or 500,000 IU D3...steer...1...d...1 for 10 d prior to harvest). Cattle (n = 466) were harvested in 2 blocks on the basis of BW with subsequent collection of carcass data. Full loins and inside rounds (n = 144 of each subprimal) were collected for fabrication of 5 steaks from the longissimus lumborum (LL), gluteus medius (GM), and semimembranosus (SM), which were aged for 7, 14, 21, 28, or 35 d. Warner- Bratzler shear force (WBSF) was used to evaluate mechanical tenderness of LL, GM, and SM steaks at all aging periods. Slice shear force (SSF) analysis was conducted on only 14- and 21-d LL steaks. No interactions (P > 0.05) between ZH and D3 occurred throughout the entire study. Supplementing ZH resulted in increased HCW (P < 0.01), larger LM area (P < 0.01), and improved calculated yield grades (P < 0.01) with decreases in fat thickness (P = 0.02) and marbling scores (P = 0.05). Supplementation with D3 increased calculated yield grade (P < 0.01) and decreased (P = 0.01) rib eye area. Feeding ZH increased (P ≤ 0.05) WBSF of LL steaks at each postmortem age interval, whereas D3 had no effect (P > 0.05) on WBSF or SSF of LL steaks. Like for WBSF, ZH supplementation increased SSF values at 14 and 21 d postmortem (P < 0.01) compared with those for non-ZH steaks. There was an interaction between ZH and postmortem age (P < 0.01) for WBSF of LL steaks. At 7 d LL steaks from ZH steers sheared over 0.6 kg greater than non-ZH steaks; however, by 21 d this difference was reduced to an average of 0.2 kg. Differences in distribution between LL steaks below 3.0 kg from non-ZH and ZH-fed cattle were also notable (P ≤ 0.05) through 21 d of aging. At 35 d postmortem a high proportion of LL steaks (68.5%) from ZH-fed steers required less than 3.0 kg to shear. Supplementation with ZH and D3 had no impact (P > 0.05) on WBSF values of GM steaks. Feeding ZH did not alter WBSF of SM steaks, but at 28 d D3 increased (P = 0.04) WBSF values. Shear force in ZH steaks was not effectively reduced by feeding D3 for 10 d to steers prior to harvest. Aging, however, was an effective method of reducing initially greater shear force values in LL steaks and, to a lesser degree, GM steaks from ZH-fed cattle.