Application of Response Surface Methodology (RSM) to optimize culture media for the production of rhamnolipids by Pseudomonas aeruginosa

Rhamnolipids, naturally existent biosurfactantsassembledbyrhamnose sugar molecules and βhydroxyalkanoicacids that has wide range of promising profitable applications. In the study Pseudomonas aeruginosabacterial isolates was identified and screened for rhamnolipid production. P. aeruginosa achieved the production of rhamnolipid at room temperature in static cultures of a mineral salts medium containing olive oil. The pure culture was enhanced and optimized at pH-7, temperature-37°C and olive oil as effective carbon source. The cultural media was optimized using response surface methodology -Box-Behnken with 30 ml of olive oil, 5 g/l NaNO3, 4 g/l KH2PO4 and 7.5 g/l FeSO4 mixture contributes maximum production of rhamnolipid. The R2 value was 99.8 that correlated with adjusted R2 value of 99.7 and predicted R2 value of 99.5 shows the model is significant.