892. GeneJammer® Enhances Cellular Transduction with Recombinant Adenovirus

Viral vectors are extensively used to deliver exogenous DNA in eukaryotic cells for a broad range of applications from basic research to potential clinical uses. Adenoviruses are particularly attractive vectors due to their ability to infect a wide range of cell types. A limiting step in recombinant adenovirus uptake in certain cell types is the internalization into the target cells which is mediated by membrane receptors. Although polyamines have been widely studied regarding their ability to enhance transfer of DNA into cells, little is known about their potential role in viral transduction of cells.In this study, we have investigated the ability of GeneJammer® transfection reagent (Stratagene, La Jolla, CA) to enhance in vitro adenoviral transduction efficiency of cell types lacking adenoviral receptors. When GeneJammer® was present during transduction of human bone marrow mesenchymal stem cells (hBM-MSC) with an adenovirus type 5 carrying the eGFP gene (Ad5eGFP), infection efficiency was enhanced (82%) compared with that in the control without the polyamine (p