Optimisation and characterisation of enzyme-linked immunosorbent assay (ELISA) for the detection of the Acremonium loliae endophyte in Lolium perenne

An antigen of Acremonium loliae Latch, Christensen and Samuels can be solubilised from dried ryegrass samples by resuspending ground plant tissue in buffer containing Tween 20 (0.2%) at 5°C. Additional ovalbumin coating increases the antigenic reactivity of samples and decreases replicate variance....

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Veröffentlicht in:	New Zealand journal of agricultural research 1986, Vol.29 (1), p.117-120
Hauptverfasser:	Musgrove, D.R, Fletcher, L.R
Format:	Artikel
Sprache:	eng
Schlagworte:	Acremonium Acremonium loliae antigens Biological and medical sciences detection ELISA endophytes enzyme-linked immunosorbent assay Fundamental and applied biological sciences. Psychology Fungal plant pathogens Generalities. Techniques Lolium perenne optimization Phytopathology. Animal pests. Plant and forest protection
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Zusammenfassung:	An antigen of Acremonium loliae Latch, Christensen and Samuels can be solubilised from dried ryegrass samples by resuspending ground plant tissue in buffer containing Tween 20 (0.2%) at 5°C. Additional ovalbumin coating increases the antigenic reactivity of samples and decreases replicate variance. This antigen solubilisation procedure is as effective in detecting the A. Loliae antigen as grinding samples with a pestle and mortar, and is less time consuming.
ISSN:	0028-8233 1175-8775
DOI:	10.1080/00288233.1986.10417983