Antioxidant Properties of ADJ6 and ITS Effect on Isolated Human Erythrocyte Ghost Membrane

The present study focuses to study the ameliorative effect of ADJ6 against oxidative stress on human erythrocytes. The membrane stabilization activity, Inhibition of lipid peroxidation and the effect against Ouabain induced Na^sup +^ K^sup +^ ATPase activity was assessed. Further, in vitro antioxidant activity of ADJ6 was also determined. DPPH radical scavenging activity, metal chelating and H^sub 2^O^sub 2^ radical scavenging activity were IC^sub 50^=37.9 ± 0.22 μg/ml, IC^sub 50^=53.95 ± 0.337 μg/ml and IC^sub 50^=69.5 ± 0.628 μg/ml respectively. ADJ6 showed an EC^sub 50^=90.83 ± 1.905 μg/ml against hypotonicity induced lysis of erythrocytes. H2O2 induced Lipid peroxidation of ghost membrane was also inhibited by ADJ6 (IC^sub 50^=84.5 ± 2.613 μg/ml). Ouabain induced sodium potassium ATPase activity increased with the treatment of ADJ6 in a dose dependent manner. This proves that ADJ6 is effective in stabilizing the membrane potential of erythrocyte membrane. The scavenging activity in vitro also proves that ADJ6 is effective against free radicals.