High Performance Thin Layer Chromatography and High Performance Liquid Chromatography determination of Quercetin from Polyalthia longifolia leaves

Polyalthia longifolia Thwaites is an ornamentally important flowering tree with immense potential. Identification of phytochemical constituents from plant extracts is need of hour in developing countries like India. These elucidated constituents may play a pivotal role in providing remedies to plethora of diseases whether major or minor. Proper application of analytical technique is key to success in interpretation of these plant constituents. In present research studies the aim was to find out by chromatographic techniques how much was percentage of quercetin present in the Polyalthia longifolia Thwaites leaves ethanolic extract. Polyalthia longifolia Thwaites leaves after ethanolic extraction and preliminary phytochemical studies were subjected to identification of quercetin by high performance thin layer chromatography and high-pressure liquid chromatography. Preliminary phytochemical evaluation confirmed the presence of flavonoids. The active constituent identified from Polyalthia longifolia Thwaites leaves extract was found to be identical to quercetin.