STELLATE GANGLION ELECTRICAL STIMULATION FOR ESTABLISHING A CANINE MODEL OF ACUTE ATRIAL FIBRILLATION MEDIATED BY SYMPATHETIC NERVE

Objectives To build the methodology of acute animal model of atrial fibrillation (AF) induced by increased sympathetic nerve activity. Methods Sixteen adult mongrel dogs weighing 18 to 25 kg were randomly divided into 3 groups. Control group (n=4) underwent 6-h rapid atrial pacing only. RSG group (n=6) underwent 6-h right stellate ganglion (RSG) stimulation plus rapid atrial pacing. LSG group (n=6) underwent 6-h left stellate ganglion (LSG) electrical stimulation plus rapid atrial pacing. AF induction rate, and AF duration in left atrium (LA), right atrium (RA), left superior pulmonary vein (LSPV) and left inferior pulmonary vein (LIPV) sites were measured. Results (1) We successfully established canine models of acute AF induced by increased sympathetic nerve activity. The methods were relatively simple and repeatable. The AF duration was relatively long. (2) The effect on AF inducibility: In RSG group, the induction rate of AF was significantly increased in RA sites (73.30% vs 25.00%, p