Effect of pyridoxamine and telmisartan on tubular epithelial cells proliferation and its mechanism

Objective The purpose of this study was to explore the protective effects of telmisartan and pyridoxamine on tubular epithelial cells (HK-2) in early renal damage. Methods Cultured HK-2 cells were divided into HK-2 control, angiotensin II (10−6 mol/l) group (Ang II), telmisartan (10−6 mol/l, T) group, pyridoxamine group (1 mmol/l, P1), pyridoxamine group (10 mmol/l, P10), and T (10−6 mol/l)+P (10 mmol/l) group (T+P). Methyl thiazolyl tetrazolium (MTT) was used to measure for cell proliferation. Reactive oxygen species (ROS) generation in cellular supernatant was detected by flow cytometry. Real-time quantitative PCR was performed to measure the mRNA expression of transforming growth factor β1 (TGFβ1) and receptor for advanced glycation end-products (RAGE). Results The OD values of HK-2 were inhibited in Ang II (p