A study the relation of ACE gene polymorphisms and risk factor with coronary heart disease in Hainan Li and Han nationality

Objective To probe into the relation of the angiotensin converting enzyme (ACE) gene polymorphisms and the risk factor with coronary heart disease (CHD) in the Hainan Li and Han nationality. Methods Used the polymerase chain reaction (PCR) to detect the polymorphisms of ACE gene insertion/deletion (I/D) in 150 patients with CHD and 150 healthy people from Hainan Li and Han nationality. The genotype frequencies and allele frequencies of DD, DI and II were observed. Specific PCR detection was performed for patients who have been determined as DD by normal PCR to reduce misclassification rate. Results In the CHD group of Hainan Li nationality, the genotype frequencies of DD, DI and II were 24.7%, 32.7%, 42.6% respectively, and the allele frequencies of D and I were 41.0% and 59.0%. In the healthy control of Hainan Li nationality, the genotype frequencies of DD, DI and II were 14.0%, 44.0%, 42.0% respectively, and the allele frequencies of D and I were 36.0% and 64.0%. There were significant differences both in the genotype frequencies of DD, DI and II, and in allele frequencies D and I between these two groups (p