Effects of Ginsenosides-Rbl inhibiting cardiomyocyte hypertrophy via NHE-1-dependent calcineurin activation ex vivo

Objectives Ginsenosides-Rbl (Gs-Rbl), a main component of Ginsenosides extracted from Ginseng, is a type of activity element playing a therapeutic role in the cardiovascular system. The present study aimed at determining whether Gs-Rbl exerts a direct antihypertrophic effect and the potential underlying mechanisms on cultured cardiomyocytes ex vivo. Methods Neonatal rat ventricular cardiomyocytes were randomly divided into control group, simple phenylephrine (PE, 5 umol/l, α1 adrenoceptor agonist) group and Gs-Rb1 group (on the basis of PE intervention) for 36 h; The concentration of Gs-Rb1 is 50 umol/l, 100 umol/l, 200 umol/l and 500 umol/l, respectively. Cell surface area, Na+-H+ exchanger 1 (NHE-1), and NFAT3 was analysed. Results Phenylephrine (PE) leaded to a marked hypertrophic effect on neonatal cardiomyocytes (847±20 m2 to 1147±30 m2, p=0.000); Gs-Rb1 attenuated hypertrophic effect of PE in a concentration-dependent manner (p