e0525 Protective effect of simvastatin combined with anisodamine on myocardial perfusion in swine no reflow model

Objectives To evaluated the preventive effect of simvastatin combined with anisodamine on myocardial perfusion in no reflow, and to probe the possible mechanism. Method Totally 16 minipig of 30–40 Kg were randomly divided into anisodamine groups (A, n=8) and anisodamine plus simvastatin group (A+S, n=8). Pigs in Group A+S were pretreated with oral simvastatin for 7 days, while pigs in A groups were given placebo. Seven days later, CAG was performed, and the dopper wire was used to record blood velocity. The pressure of aorta (Pa) was monitored. PMBS was injected to establish no reflow model. Anisodamine was injected into the LAD 2 min before PBMS was injected. The TIMI blood flow, TMPG and CTFC were recorded to evaluate the myocardial perfusion. The sample of myocardium in ischaemic zone and normal zone were measured. Blood sample was taken before and after the experiment to measure the level of CK-MB, cTnI and hs-CRP. The percent of necrotic myocardium was calculated by myocardium stain method. Results The TIMI blood flow and TFCs were better in Group A+S (p