e0198 Activity of calcineurin NFAT signalling pathway involving in remodelling in cTnI R146W mice

Objective To construct a transgenic model of HCM overexpressing cTnIR146W, observe the pathological change of this animal, and elucidate the signalling pathway involving in heart remodelling of cTnIR146W +/− mice. Methods Cardiac hypertrophy-related signalling pathway protein, such as calcineurin, calsarcin-1, GSK-3β, AKT, SERCA2, PLB were detected by Western blot and RT-PCR. We also assessed the activity of calcineurin in cTnIR146W +/− mice, in order to elucidate potential mechanisms involving in the cardiac remodelling in cTnIR146W +/− mice. Results The total expression of cTnI in cTnIR146W +/− mice was significant higher than cTnIR146W −/− mice (p