SAT0525 Environmental Factors Associated with Paget's Disease of Bone or with the Sqstm1/P392l Mutation Carriage

Background Several publications have demonstrated that the most frequent mutation in Paget's disease of bone (PDB), SQSTM1/P392L, leads to some of the phenotypic characteristics of PDB, but this single mutation is seemingly unable to result in the complete pagetic phenotype, suggesting that other mechanisms such as environmental factors may play a role. Objectives Identify environmental factors associated with PDB or with the SQSTM1/P392L mutation carriage in the French-Canadian population. Methods We investigated environmental factors through a questionnaire in 176 French-Canadian patients with PDB and 147 healthy controls not carrier of the SQSTM1/P392L mutation. The questionnaire contained the following sections: socio-demographic and physical characteristics, tobacco exposure, diet, residency, work, leisure, and contact with animals, during childhood/adolescence and adulthood. Associations between environmental factors and PDB, or SQSTM1/P392L mutation (86 individuals carried the mutation, including 48 patients with PDB), were searched relying on Chi-squared, Fisher and t tests. Odds ratio (OR) and 95% confidence interval were calculated. Univariate followed by multivariate analysis were done for association with PDB. Correlation between members of same family was considered when possible. Analysis adjusted for age and gender were done for the association study with SQSTM1/P392L mutation. Results We administered the questionnaire to 361 participants: 176 patients with PDB and 147 healthy controls. 86 individuals were carriers of the SQSTM1/P392L mutation, among whom 48 were patients with PDB and 38 were healthy carriers of the mutation. In univariate model, we found an association of PDB with, wood-fire heating in childhood (OR=2.48 (1.37-4.49), p