FRI0023 Enzymatic dnase and hyaluronidase serum activity in patients with early arthritis

Background Early arthritis (EA) is one of the most important problems of modern rheumatology. Searching the new pathogenic mechanism of recently-onset joints inflammation caused by different factors is needed. Enzymatic serum activity is multifactorial system, which play major role in inflammation processes in rheumatic diseases. The aim of our study was to study the levels of nuclease (DNAse) and hyaluronidase activity in patients with EA and to evaluate their pathogenic and diagnostic significance. Objectives We studied 190 persons: 64 patients with early rheumatoid arthritis (ERA) (disease duration 4.23±2.21 months), 55 patients with acute reactive arthritis (AReA), associated with Chlamydia trachomatis urogenital infection(disease duration 3.15±2.11 months), 36 patients with unclassified arthritis (UA) (disease duration 3.32±2.14 months) and 40 healthy controls from the same regional area in Belarus. Methods The samples of patients and healthy persons sera were investigated. DNAse serum activity was determined by rivanol clot prevention test and agarose electrophoresis. Hyaluronidase serum activity was determined by rivanol clot prevention test. Statistical data differences for means and medians were assessed by the Student’s t-test and Mann-Whitney’s U-test, respectively. Statistical correlations were calculated by the Spearman method. Results The levels of DNAse serum activity in patients with EA - ERA (Me 4.50 units, 95%CI 4.00-5.00), AReA (Me 3.00 units 95%CI 2.92-3.00), UA (Me 3.00 units, 95%CI 2.00-4.00) were higher (p