Molecular Reference Materials for Standardization of Allergen Measurements

Methods Natural and recombinant food, dust mite, pollen and animal allergens (n = 11) were purified by affinity and/or gel-filtration chromatography and analyzed by SDS-PAGE, LC-MS/MS, ELISA, and FEIA or chimeric IgE ELISA.

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Veröffentlicht in:Journal of allergy and clinical immunology 2016-02, Vol.137 (2), p.AB24-AB24
Hauptverfasser: Wünschmann, Sabina, PhD, Prtorich, Kristie N, Minichino, Cathy, Cerritos, Heaven, Vailes, Lisa D, Chapman, Martin D., PhD, FAAAAI
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Sprache:eng
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Zusammenfassung:Methods Natural and recombinant food, dust mite, pollen and animal allergens (n = 11) were purified by affinity and/or gel-filtration chromatography and analyzed by SDS-PAGE, LC-MS/MS, ELISA, and FEIA or chimeric IgE ELISA.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2015.12.079