Intracellular Expression of Fluorochrome Labelled-Diamine Oxidase in Basophils: A Novel Diagnostic Tool for Peanut Allergy

Methods Basophil activation and histamine release was measured in peanut allergic patients (n=10), peanut sensitized (n=4) undergoing an oral food challenge and non-atopic controls (n=10) by expression of CD63 and intracellular DAO by flow cytometry.

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Veröffentlicht in:Journal of allergy and clinical immunology 2016-02, Vol.137 (2), p.AB137-AB137
Hauptverfasser: Mohseni, Yasmin R., BSc, Turner, Paul J., FRACP, PhD, Boyle, Robert J., MBChB, PhD, Clark, Andrew, MRCPCH, MD, Robb, Abigail O., BSc, Durham, Stephen R., MA, MD, FRCP, Shamji, Mohamed H., BSc, MSc, PhD, FAAAAI
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Sprache:eng
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Zusammenfassung:Methods Basophil activation and histamine release was measured in peanut allergic patients (n=10), peanut sensitized (n=4) undergoing an oral food challenge and non-atopic controls (n=10) by expression of CD63 and intracellular DAO by flow cytometry.
ISSN:0091-6749
1097-6825
DOI:10.1016/j.jaci.2015.12.583