Inhibitory Effect of Fluoride on Na^sup +^,K^sup +^ ATPase Activity in Human Erythrocyte Membrane

Inhibitory Effect of Fluoride on Na^sup +^,K^sup +^ ATPase Activity in Human Erythrocyte Membrane

The present study was performed to evaluate the role of long-term consumption of excessive fluoride on electrolyte homeostasis and their transporting mechanisms in erythrocytes of subjects afflicted with dental and skeletal fluorosis. A total of 620 adult (20-50 years) Indian residents participated...

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Veröffentlicht in:Biological trace element research 2015-12, Vol.168 (2), p.340
Hauptverfasser: A, Shashi, G, Meenakshi
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Enzymes
Erythrocytes
Fluorides
Ions
Membranes
Regression analysis
Sodium
Trace elements
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description The present study was performed to evaluate the role of long-term consumption of excessive fluoride on electrolyte homeostasis and their transporting mechanisms in erythrocytes of subjects afflicted with dental and skeletal fluorosis. A total of 620 adult (20-50 years) Indian residents participated in this study: 258 men and 242 women exposed to high concentrations of fluoride and 120 age and gender-matched control subjects. Erythrocytes were isolated from blood samples, washed, and used for the estimation of intraerythrocyte sodium and potassium concentrations. Na^sup +^,K^sup +^ ATPase activity was determined spectrophotometrically from a ghost erythrocyte membrane prepared by osmotic lysis. Erythrocyte analytes were correlated with the water and serum fluoride concentrations by Pearson's bivariate correlation and regression analysis. Results indicated a significant increase in intraerythrocyte sodium (F=14306.265, P
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A total of 620 adult (20-50 years) Indian residents participated in this study: 258 men and 242 women exposed to high concentrations of fluoride and 120 age and gender-matched control subjects. Erythrocytes were isolated from blood samples, washed, and used for the estimation of intraerythrocyte sodium and potassium concentrations. Na^sup +^,K^sup +^ ATPase activity was determined spectrophotometrically from a ghost erythrocyte membrane prepared by osmotic lysis. Erythrocyte analytes were correlated with the water and serum fluoride concentrations by Pearson's bivariate correlation and regression analysis. Results indicated a significant increase in intraerythrocyte sodium (F=14306.265, P<0.0001) in subjects from endemic fluorosis study groups as compared to controls. A significant (P<0.05) positive correlation of intracellular sodium was found with water and serum fluoride concentrations. Mean concentration of intraerythrocytic potassium ions showed significant reduction (F=9136.318, P<0.0001) in subjects exposed to fluoride. A significant (P<0.05) negative correlation of potassium ions was noted with water and serum fluoride concentrations. Na^sup +^,K^sup +^ ATPase activity was significantly declined (F=1572.763, P<0.0001) in subjects exposed to fluoride. A significant (P<0.05) inverse relationship of Na^sup +^,K^sup +^ ATPase activity was revealed with water and serum fluoride concentrations.]]></description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/s12011-015-0349-9</identifier><language>eng</language><publisher>Clifton: Springer Nature B.V</publisher><subject>Enzymes ; Erythrocytes ; Fluorides ; Ions ; Membranes ; Regression analysis ; Sodium ; Trace elements</subject><ispartof>Biological trace element research, 2015-12, Vol.168 (2), p.340</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>A, Shashi</creatorcontrib><creatorcontrib>G, Meenakshi</creatorcontrib><title>Inhibitory Effect of Fluoride on Na^sup +^,K^sup +^ ATPase Activity in Human Erythrocyte Membrane</title><title>Biological trace element research</title><description><![CDATA[The present study was performed to evaluate the role of long-term consumption of excessive fluoride on electrolyte homeostasis and their transporting mechanisms in erythrocytes of subjects afflicted with dental and skeletal fluorosis. 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Mean concentration of intraerythrocytic potassium ions showed significant reduction (F=9136.318, P<0.0001) in subjects exposed to fluoride. A significant (P<0.05) negative correlation of potassium ions was noted with water and serum fluoride concentrations. Na^sup +^,K^sup +^ ATPase activity was significantly declined (F=1572.763, P<0.0001) in subjects exposed to fluoride. 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subjects Enzymes
Erythrocytes
Fluorides
Ions
Membranes
Regression analysis
Sodium
Trace elements
title Inhibitory Effect of Fluoride on Na^sup +^,K^sup +^ ATPase Activity in Human Erythrocyte Membrane
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