Inhibitory Effect of Fluoride on Na^sup +^,K^sup +^ ATPase Activity in Human Erythrocyte Membrane

The present study was performed to evaluate the role of long-term consumption of excessive fluoride on electrolyte homeostasis and their transporting mechanisms in erythrocytes of subjects afflicted with dental and skeletal fluorosis. A total of 620 adult (20-50 years) Indian residents participated...

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Veröffentlicht in:Biological trace element research 2015-12, Vol.168 (2), p.340
Hauptverfasser: A, Shashi, G, Meenakshi
Format: Artikel
Sprache:eng
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Zusammenfassung:The present study was performed to evaluate the role of long-term consumption of excessive fluoride on electrolyte homeostasis and their transporting mechanisms in erythrocytes of subjects afflicted with dental and skeletal fluorosis. A total of 620 adult (20-50 years) Indian residents participated in this study: 258 men and 242 women exposed to high concentrations of fluoride and 120 age and gender-matched control subjects. Erythrocytes were isolated from blood samples, washed, and used for the estimation of intraerythrocyte sodium and potassium concentrations. Na^sup +^,K^sup +^ ATPase activity was determined spectrophotometrically from a ghost erythrocyte membrane prepared by osmotic lysis. Erythrocyte analytes were correlated with the water and serum fluoride concentrations by Pearson's bivariate correlation and regression analysis. Results indicated a significant increase in intraerythrocyte sodium (F=14306.265, P
ISSN:0163-4984
1559-0720
DOI:10.1007/s12011-015-0349-9