Mass production of high quality corneal endothelial cells from old donors

Purpose In vitro expansion of human corneal endothelial cells (CECs) possessing differentiation status and high endothelial cell density (ECD) is the key for the future cell therapies addressing endothelium dysfunctions. A few recent successes were reported using young donors (2500 cells/mm2) was obtained. Alizarin red staining showed typical morphology of CECs. Conclusions This new protocol provided a very high quality of cultured CECs from old donors. The satisfying yield and high ECD allows us to plan its transfer to a clinical grade process.