Evaluation of predictive CYP2C19 genotyping assays relative to measured phenotype in a South African cohort
To align predicted and measured CYP2C19 phenotype in a South African cohort. Genotyping of CYP2C19*2, *3, *9, *15, *17, *27 and *28 was performed using PCR-RFLP, and an activity score (AS) system was used to predict phenotype. True phenotype was measured using plasma concentrations of omeprazole and...
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Veröffentlicht in: | Pharmacogenomics 2015-08, Vol.16 (12), p.1343-1354 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | To align predicted and measured CYP2C19 phenotype in a South African cohort.
Genotyping of CYP2C19*2, *3, *9, *15, *17, *27 and *28 was performed using PCR-RFLP, and an activity score (AS) system was used to predict phenotype. True phenotype was measured using plasma concentrations of omeprazole and its metabolite 5'-hydroxyomperazole.
Partial genotype-phenotype discrepancies were reported, and an adapted AS system was developed, which showed a marked improvement in phenotype prediction. Results highlight the need for a more comprehensive CYP2C19 genotyping approach to improve prediction of omeprazole metabolism.
Evidence for the utility of a CYP2C19 AS system is provided, for which the accuracy can be further improved by means of comprehensive genotyping and substrate-specific modification. |
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ISSN: | 1462-2416 1744-8042 |
DOI: | 10.2217/PGS.15.80 |