Study on Multiplication, Rooting and Transplanting of Tissue Culture Plantlets of Rhododendron chrysanthum Pall

This study aimed to shorten the multiplication culture and rooting culture periods of Rh. chrysarithum Pall. The Rh. chrysanthum Pall tissue culture plantlets collected from Changbai Mountain were used as material, and the effects of different hormone combinations and coconut milk on the proliferation and differentiation of Rh. chrysanthum Pall tissue culture plantlets were investigated. In addition, the rooting medium and transplanting matrix for Rh. chiysanthum Pall tissue culture plantlets were explored. The medium composed of modified MS, IBA and ZT was the optimum medium for subculture multiplication of Rh. chiysanthum Pall tissue culture plantlets. The multiplication multiple and average plant height were significantly improved by adding coconut milk into the medium. For Rh. chrysanthum Pall tissue culture plantlets, the optimum rooting culture medium was composed of modified MS and IBA, and the tissue culture plantlets began to root 8 d after the inoculation. The root induction treatment was carried out after a 15-d sand culture, and the suitable matrix was composed of turfy soil, humus soil and perlite.