ULTRAVIOLET AND MICROWAVE RADIATION IN TRICHODERMA VIRIDE ISOLATES

This paper aimed to determine the exposition time close to 5% ofsurvival of Trichoderma viride (TSM) under ultraviolet light, and TSM andIT isolates of Trichoderma viride to microwave radiation, in order to obtainthe mutants resistant to benomyl fungicide. This survival was studied fromthe irradiation of conidia at different exposition times. 0.1mL aliquots ofconidia suspension from the isolates were irradiated at time of 5% of survivaland incubated in PDA medium supplemented with doses of 0; 1.0; 5.0; and10 g.mL-1 of benomyl. The results showed that the exposition time ofthe TSM isolate to ultraviolet radiation was about two minutes and formicrowave radiation the time was eight seconds for both TSM and ITisolates. Conidia from IT isolate were more resistant to microwave radiationthan the TSM isolate. Eight resistant mutants to 5.0 g.mL-1 benomylfungicide were obtained from the TSM isolate exposed to ultravioletradiation, both according to the resistance to the fungicide and theantagonism in vitro to phytopathogens, though with different colors ofmycelia. Colonies of mutants resistant to benomyl with the use ofmicrowave radiation were not observed.