In Vitro Activities of Moxifloxacin, ^sup 127^I-Moxifloxacin and ^sup 131^I-Moxifloxacin Against Staphylococcus Aureus Biofilms

The aim of the study was to investigate the antimicrobial effect of Moxifloxacin (MXF), radio (Na^sup 131^I) and cold (K^sup 127^I) iodinated MXF on methicillin susceptible Staphylococcus aureus ATCC 35556 (MSSA) biofilms. MXF was labeled with Na^sup 131^I using the iodogen method. The optimum radiodination conditions for ^sup 131^I-MXF was determined by thin-layer radio chromatography studies. Thin-layer radio chromatography (TLRC) chromatograms were obtained by using Cyclone Plus Storage Phosphor System. MXF was labeled with ^sup 131^I iodogen method. ^sup 131^I-MXF was obtained with high a yield 95±3%. The MIC values for MXF, ^sup 127^I-MXF and ^sup 131^I-MXF was 0.06 μg/mL. Bactericidal activity was demonstrated at 0.25 μg/mL 4 hour for MXF, ^sup 127^I-MXF and ^sup 131^I-MXF. At MIC levels, MXF, ^sup 127^I-MXF and ^sup 131^I-MXF was not showed a marked reduction of metabolic activity in the S. aureus biofilm. The ODs of biofilm after incubation with an increasing antibiotic concentration were significantly lower than the ODs of biofilms without antibiotic p≤005.