Simultaneous Vision-Based Shape and Motion Analysis of Cells Fast-Flowing in a Microchannel
This paper proposes a novel concept for simultaneous cell shape and motion analysis in fast microchannel flows by implementing a multiobject feature extraction algorithm on a frame-straddling high-speed vision platform. The system can synchronize two camera inputs with the same view with only a tiny...
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| Veröffentlicht in: | IEEE transactions on automation science and engineering 2015-01, Vol.12 (1), p.204-215 |
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| container_title | IEEE transactions on automation science and engineering |
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| creator | Qingyi Gu Aoyama, Tadayoshi Takaki, Takeshi Ishii, Idaku |
| description | This paper proposes a novel concept for simultaneous cell shape and motion analysis in fast microchannel flows by implementing a multiobject feature extraction algorithm on a frame-straddling high-speed vision platform. The system can synchronize two camera inputs with the same view with only a tiny time delay on the sub-microsecond timescale. Real-time video processing is performed in hardware logic by extracting the moment features of multiple cells in 512 × 256 images at 4000 fps for the two camera inputs and their frame-straddling time can be adjusted from 0 to 0.25 ms in 9.9 ns steps. By setting the frame-straddling time in a certain range to avoid large image displacements between the two camera inputs, our frame-straddling high-speed vision platform can perform simultaneous shape and motion analysis of cells in fast microchannel flows of 1 m/s or greater. The results of real-time experiments conducted to analyze the deformabilities and velocities of sea urchin egg cells fast-flowing in microchannels verify the efficacy of our vision-based cell analysis system. |
| doi_str_mv | 10.1109/TASE.2013.2292583 |
| format | Article |
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The system can synchronize two camera inputs with the same view with only a tiny time delay on the sub-microsecond timescale. Real-time video processing is performed in hardware logic by extracting the moment features of multiple cells in 512 × 256 images at 4000 fps for the two camera inputs and their frame-straddling time can be adjusted from 0 to 0.25 ms in 9.9 ns steps. By setting the frame-straddling time in a certain range to avoid large image displacements between the two camera inputs, our frame-straddling high-speed vision platform can perform simultaneous shape and motion analysis of cells in fast microchannel flows of 1 m/s or greater. The results of real-time experiments conducted to analyze the deformabilities and velocities of sea urchin egg cells fast-flowing in microchannels verify the efficacy of our vision-based cell analysis system.</description><identifier>ISSN: 1545-5955</identifier><identifier>EISSN: 1558-3783</identifier><identifier>DOI: 10.1109/TASE.2013.2292583</identifier><identifier>CODEN: ITASC7</identifier><language>eng</language><publisher>New York: IEEE</publisher><subject>Algorithms ; Automated cell inspection ; Cameras ; cell deformation analysis ; Cells ; Delay effects ; Feature extraction ; frame-straddling camera ; Head ; High speed ; high-speed vision ; lab-on-a-chip (LOC) ; Logic ; Microchannel ; Microchannels ; multiobject feature extraction ; Platforms ; Real time ; Real-time systems ; Shape ; Vision</subject><ispartof>IEEE transactions on automation science and engineering, 2015-01, Vol.12 (1), p.204-215</ispartof><rights>Copyright The Institute of Electrical and Electronics Engineers, Inc. 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The system can synchronize two camera inputs with the same view with only a tiny time delay on the sub-microsecond timescale. Real-time video processing is performed in hardware logic by extracting the moment features of multiple cells in 512 × 256 images at 4000 fps for the two camera inputs and their frame-straddling time can be adjusted from 0 to 0.25 ms in 9.9 ns steps. By setting the frame-straddling time in a certain range to avoid large image displacements between the two camera inputs, our frame-straddling high-speed vision platform can perform simultaneous shape and motion analysis of cells in fast microchannel flows of 1 m/s or greater. The results of real-time experiments conducted to analyze the deformabilities and velocities of sea urchin egg cells fast-flowing in microchannels verify the efficacy of our vision-based cell analysis system.</description><subject>Algorithms</subject><subject>Automated cell inspection</subject><subject>Cameras</subject><subject>cell deformation analysis</subject><subject>Cells</subject><subject>Delay effects</subject><subject>Feature extraction</subject><subject>frame-straddling camera</subject><subject>Head</subject><subject>High speed</subject><subject>high-speed vision</subject><subject>lab-on-a-chip (LOC)</subject><subject>Logic</subject><subject>Microchannel</subject><subject>Microchannels</subject><subject>multiobject feature extraction</subject><subject>Platforms</subject><subject>Real time</subject><subject>Real-time systems</subject><subject>Shape</subject><subject>Vision</subject><issn>1545-5955</issn><issn>1558-3783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>RIE</sourceid><recordid>eNpdkE1Lw0AQhhdRsFZ_gHhZ8OIldb8_jrW0KrR4aPXiYdkkG7slzdZsgvTfm5DiwdMMwzPDvA8AtxhNMEb6cTNdzycEYTohRBOu6BkYYc5VQqWi533PeMI155fgKsYdQoQpjUbgc-33bdnYyoU2wg8ffaiSJxtdDtdbe3DQVjlchaYbw2lly2P0EYYCzlxZRriwsUkWZfjx1Rf0FbRw5bM6ZFtbVa68BheFLaO7OdUxeF_MN7OXZPn2_DqbLpOMCdIkhdMMy4wqREiW5QUquHacE5HmGeKaphwTxEjKpMSE5KnLhUodploKnQst6Rg8DHcPdfhuXWzM3sese3BIZbAQCEmpSI_e_0N3oa27XD3FOoQypDsKD1SXJcbaFeZQ-72tjwYj0-s2vW7T6zYn3d3O3bDjnXN_vBCKa4boL47ZehQ</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Qingyi Gu</creator><creator>Aoyama, Tadayoshi</creator><creator>Takaki, Takeshi</creator><creator>Ishii, Idaku</creator><general>IEEE</general><general>The Institute of Electrical and Electronics Engineers, Inc. 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| subjects | Algorithms Automated cell inspection Cameras cell deformation analysis Cells Delay effects Feature extraction frame-straddling camera Head High speed high-speed vision lab-on-a-chip (LOC) Logic Microchannel Microchannels multiobject feature extraction Platforms Real time Real-time systems Shape Vision |
| title | Simultaneous Vision-Based Shape and Motion Analysis of Cells Fast-Flowing in a Microchannel |
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