The Heterologous Expression of the Iris lactea var. chinensis Type 2 Metallothionein IlMT2b Gene Enhances Copper Tolerance in Arabidopsis thaliana
Iris lactea var. chinensis ( I. lactea var. chinensis ) is a widely adapted perennial species with a high level of copper tolerance. To evaluate the role of metallothioneins (MTs) in copper tolerance in I. lactea var. chinensis , a full-length cDNA homologue of MT2, designated IlMT2b (GenBank access...
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Veröffentlicht in: | Bulletin of environmental contamination and toxicology 2015-02, Vol.94 (2), p.247-253 |
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Sprache: | eng |
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Zusammenfassung: | Iris lactea
var.
chinensis
(
I. lactea
var.
chinensis
) is a widely adapted perennial species with a high level of copper tolerance. To evaluate the role of metallothioneins (MTs) in copper tolerance in
I. lactea
var.
chinensis
, a full-length cDNA homologue of MT2, designated
IlMT2b
(GenBank accession No. AB907788), was cloned using the RACE-PCR method. The expression level of
IlMT2b
in the leaves and roots of
I. lactea
var.
chinensis
was induced in response to copper (Cu) treatment. Ectopic expression of
IlMT2b
in
Arabidopsis thaliana
increased the Cu concentration and reduced H
2
O
2
production in the transgenic plants. After treatment with 50 and 100 μM Cu, the root length of two transgenic seedlings was respectively about 1.5- and 3-fold longer than that of the wild-type. Together, these results suggested that
IlMT2b
may represent a useful target gene for the phytoremediation of Cu-polluted soil. |
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ISSN: | 0007-4861 1432-0800 |
DOI: | 10.1007/s00128-014-1444-x |