GW25-e5306 Hydroxysafflor yellow A protects against the particulate matter (PM2.5) -induced cell injury by modulating intracellular redox via Nrf2/Keap1 pathway in Human umbilical vein endothelial cells

GW25-e5306 Hydroxysafflor yellow A protects against the particulate matter (PM2.5) -induced cell injury by modulating intracellular redox via Nrf2/Keap1 pathway in Human umbilical vein endothelial cells

Methods Cytotoxic effects of the organic extract of PM2.5 on HUVECs were measured by means of HUVEC cell viability and the generation of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) after stimulation with PM2.5 and HSYA for the indicated concontration, Expression of heme oxy...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of the American College of Cardiology 2014-10, Vol.64 (16), p.C32-C32
Hauptverfasser: Caiping, Li, Lv, Ji-yuan
Format: Artikel
Sprache:eng
Schlagworte:
Cardiology
Cardiovascular
Internal Medicine
Medical research
Proteins
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Methods Cytotoxic effects of the organic extract of PM2.5 on HUVECs were measured by means of HUVEC cell viability and the generation of intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) after stimulation with PM2.5 and HSYA for the indicated concontration, Expression of heme oxygenase-1 (HO-1) and Nuclear factor-erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associated protein 1 (Keap1) were investigated by RT-PCR and Western blotting assays and Nrf2 translocation was assayed.
ISSN:0735-1097
1558-3597
DOI:10.1016/j.jacc.2014.06.153